Malolina E. A. (N.K. Koltzov Institute of Developmental Biology of the RAS (Moscow);
Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation (Moscow))
Kulibin A. Yu. (N.K. Koltzov Institute of Developmental Biology of the RAS (Moscow))
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A combination of small-molecule compounds, Rho-associated kinase inhibitor Y-27632 (Y), type 1 transforming growth factor-b receptor inhibitor A-83-01 (A), and glycogen synthase kinase-3 inhibitor CHIR99021 (C) (YAC), allows for the stable culturing of different types of stem and progenitor cells. Here, the effect of YAC on the culture of Sertoli-like cells from the adult mouse testis was studied using laser microdissection and RT-PCR. Some features of Sertoli-like cells make it possible to define them as progenitor cells. It was found that YAC significantly upregulates expression of genes involved in Sertoli cell specification (Wt1, Sox9 and Dmrt1) and function (Trf, Gdnf and Kitl).
Keywords:testis, Sertoli cells, small-molecule compounds, progenitor cells
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Citation link:
Malolina E. A., Kulibin A. Y. A combination of small-molecule inhibitors YAC upregulates expression of Ssertoli-cell markers in the culture of Sertoli-like cells from the adult mouse testis // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2018. -№12. -С. 36-39 |
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