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Application of the reaction of the growth of phages titer for detection

Ryskaliyeva B. Zh.  (FSBEI HE "Ulyanovsk State Agrarian University Named After P.A. Stolypin")

Vasilyev D. А.  (FSBEI HE "Ulyanovsk State Agrarian University Named After P.A. Stolypin")

Feoktistova N. А.  (FSBEI HE "Ulyanovsk State Agrarian University Named After P.A. Stolypin")

Lyashenko E. A.  (FSBEI HE "Ulyanovsk State Agrarian University Named After P.A. Stolypin")

The article shows the results of studies using the phage titer increasing reaction to detect the phytopathogenic bacterium Pectobacterium carotovorum, which causes soft rot and black leg of potato. In seed potatoes contaminated with bacteria of the genus Pectobacterium, using the phage titer increase reaction, the studied bacteria are detected in the amount of 103 microbial cells per 1 g without isolation of pure culture, in the presence of extraneous microflora. The data obtained during the study confirm that for the detection of bacteria of the genus Pectobacterium, the use of the phage titer increase reaction is an effective method that does not require isolation of a pure culture and allows to reduce the duration of the study to detect them.

Keywords:phytopathogenic bacteria, Pectobacterium carotovorum, identification, phage titer increase reaction, bacteriophage, soft rot.
 

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Citation link:
Ryskaliyeva B. Z., Vasilyev D. А., Feoktistova N. А., Lyashenko E. A. Application of the reaction of the growth of phages titer for detection // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2020. -№03/2. -С. 26-28
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