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B. petriiDevelopment of a differential diagnostic medium for identification of bacteria of the species B. petrii

Lomakin A. A.  (postgraduate, P.A. Stolypin Ulyanovsk State Agricultural University)

Vasilev D. A.  (Doctor of Sciences (Biology), professor, P.A. Stolypin Ulyanovsk State Agricultural University )

Mastilenko A. V.  (PhD in Biological sciences, associate professor, P.A. Stolypin Ulyanovsk State Agricultural University)

Schestakow A. G.  (PhD in Biological sciences, associate professor, P.A. Stolypin Ulyanovsk State Agricultural University)

The article is devoted to the development of the development of a differential diagnostic medium for the identification of bacteria of the species Bordetella petrii. The developed selective medium allows the identification of B. petrii bacteria within 48-72 hours. The developed nutrient media are specific in relation to bacteria-associates of other species and genera. The optimal composition of the medium is (per liter): sodium gluconate -20 g, casein hydrolyzate-3 g, yeast extract-1 g, monosubstituted potassium phosphate -1 g, bromothymol blue-0.08 g, bacteriological agar-15 g, cephalexin-0 02 g. The indicator of the medium sensitivity is about 100 microbial cells.

Keywords:Bordetella, B. petrii. differential diagnostic environment, identification, substrat
 

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Citation link:
Lomakin A. A., Vasilev D. A., Mastilenko A. V., Schestakow A. G. B. petriiDevelopment of a differential diagnostic medium for identification of bacteria of the species B. petrii // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2020. -№11. -С. 21-26 DOI 10.37882/2223-2966.2020.11.23
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