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Use of genomic SSR markers, RAPD and ISSR-analysis to assess the genetic diversity of dandelion officinalis

Bisultanova Zura   (Art. Lecturer, Chechen State University named after A.A. Kadyrov)

Atsaeva Maret   (Candidate of Biological Sciences, Associate Professor, Chechen State University named after A.A. Kadyrov)

Dzhambetova Petimat   (Doctor of Biol. Sci., Professor, Chechen State University named after A.A. Kadyrov)

The article describes the mechanism of using genomic SSR markers, RAPD and ISSR in order to assess the genetic diversity of dandelion officinalis. A brief description of this plant is given and the regions of its distribution are described. It is noted that the plant was previously actively used in folk medicine, which is largely due to its therapeutic and other benefits. When studying genomic markers, the significance of these studies is noted on the example of studying the patterns of genetic variation in plants, which make it possible to draw conclusions about the adaptation of plants and the identification of their genes. Describes the genomic study of dandelion officinalis using DNA extraction and RAPD analysis in laboratory conditions. When studying three plant specimens, it was found that there were no significant differences in the amplified fragments in three plant species (collected from different heights). A conclusion was also formulated that there is no relationship between the height at which a dandelion grows and its genetic variability. However, there were differences in the pattern of polymorphic loci, a difference in the size of the amplified bands. This made it possible to draw a conclusion about the effectiveness of the methods used to study interspecies differences.

Keywords:Dandelion officinalis, research, differences, genetic variability, RAPD, ISSR, marker, DNA.
 

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Citation link:
Bisultanova Z. , Atsaeva M. , Dzhambetova P. Use of genomic SSR markers, RAPD and ISSR-analysis to assess the genetic diversity of dandelion officinalis // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2022. -№01. -С. 12-16 DOI 10.37882/2223-2966.2022.01.08
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