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Method for molecular genediagnostic of group C/G streptococci

Abashin Ilya Yurievich  (postgraduate student, laboratory assistant of the Federal State Budgetary Institution “Federal Scientific Center-All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences”, Russia, Moscow)

Kozyreva Natalia Gennadievna  (cand. biol. Sci., Leading Researcher, Federal State Budgetary Institution “Federal Scientific Center-All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences”, Russia, Moscow)

Gorbatov Alexander Veniaminovich  (cand. veterinarian. sciences, acting Head of the Laboratory of Microbiology with the Museum of Type Cultures of the Federal State Budgetary Institution “Federal Scientific Center-All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences”, Russia, Moscow)

Avduevskaya Natalia Nikolaevna  (Applicant for the degree of science, researcher of the Vologda branch of the Federal State Budgetary Scientific Institution Federal Scientific Center of VIEV RAS, Russia, Vologda)

In this mini-review we considered the applied method for identifying the emm-gene in streptococci of groups C and G isolated from cattle, based on polymerase chain reaction (PCR), is a fast and accurate method for diagnosing the pathogen. The technique is based on the uniqueness of the nucleotide sequence of the pathogen, which gives a high degree of specificity. The relevance of the developed method is justified by the lack of information about the exact properties of the M-like protein of streptococci encoded by the emm-gene, which is a cellular surface-anchored protein and which presumably causes resistance to phagocytosis. For veterinary medicine, this is a new approach, in particular, when working with group C and G streptococci in cattle.

Keywords:Streptococci, molecular diagnostics, emm-gene, cattle, genediagnostic
 

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Citation link:
Abashin I. Y., Kozyreva N. G., Gorbatov A. V., Avduevskaya N. N. Method for molecular genediagnostic of group C/G streptococci // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2022. -№10/2. -С. 6-10 DOI 10.37882/2223-2966.2022.10-2.01
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