Bubyakin R. I. (Post-graduate student, FSFI «Federal Centre For Animal Health» (FGBI «ARRIAH», Vladimir))
Kononova S. V. (Research advisor, PhD, FSFI «Federal Centre For Animal Health» (FGBI «ARRIAH», Vladimir))
Shumilova I. N. (PhD, FSFI «Federal Centre For Animal Health» (FGBI «ARRIAH», Vladimir))
Kurnenkova E. V. (PhD, FSFI «Federal Centre For Animal Health» (FGBI «ARRIAH», Vladimir))
Trofimova E. A. (FSFI «Federal Centre For Animal Health» (FGBI «ARRIAH», Vladimir))
Kuznetsova E. G. (PhD, FSFI «Federal Centre For Animal Health» (FGBI «ARRIAH», Vladimir))
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The results of studies on the reproduction of BVDV genotype 2 (isolate "Chelyabinsk") in eukariotic subcultivated cell lines (2-10 passages) of lamb testicles, lamb kidney, goat kidney obtained as a result of trypsinization of the kidneys and testicles of lamb and goat kidney are presented. The results obtained indicate the successful reproduction of the pathogen under study in LT, LK, GK subcultivated cell lines. The activity of the virus by the fifth passage was 5.23±0.18 lg TCID50/cm3 in LT cell culture, 5.33±0.14 lg TCID50/cm3 in LK cell cultures and 5.25±0.17 lg TCID50/cm3 in GK cell culture. Accounting the problem of BVDV-free cell lines searching, the using of subcultivated cell lines with growth from primary trypsinized populations for 25-30 passages in modern conditions provide active viral material even on a production scale.
Keywords:subcultivated cell lines, BVDV (Bovine viral diarrhea virus), cytopathic effect, tissue culture infectious dose (TCID), multiplicity of infection (MOI).
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Citation link:
Bubyakin R. I., Kononova S. V., Shumilova I. N., Kurnenkova E. V., Trofimova E. A., Kuznetsova E. G. BOVINE VIRAL DIARRHEA VIRUS TYPE 2 REPRODUCTION IN EUKARYOTIC SUBCULTIVATED CELL LINES // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2024. -№01/2. -С. 7-11 DOI 10.37882/2223-2966.2024.1-2.02 |
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