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MASS SPECTROMETRIC ANALYSIS OF STRAINS OF GLANDERS AND MELIOIDOSIS AGENT RESISTANT TO VARIOUS DISINFECTANTS

Sharov Timur Nikolaevich  ( Ph.D. med. sciences, senior researcher of laboratory of proteomic analysis FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor )

Luchinin Dmitry Nikolaevich  (researcher of laboratory of arbovirus infections FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor )

Molchanova Elena Vladimirovna  (Ph.D. biol. sciences, senior researcher of laboratory of arbovirus infections FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor )

The article is devoted to the study of the effect on the mass spectra of Burkholderia pseudomallei and Burkholderia mallei strains of artificially formed resistance to widely used disinfectants: hydrogen peroxide, chloramine B, benzalkonium chloride, as well as glutaraldehyde. The aim of the work was to assess the changes that occur during the formation of resistance to certain substances in the pool of general cellular proteins of microoranism, as well as the relevance of replenishing the database with mass spectra of strains resistant to the described disinfectants. The study used 14 strains of pathogenic burkholderia, as well as disinfectant-resistant variants of these strains. 70 individual mass spectra were obtained and processed. A cluster analysis was carried out using the Saramis Premium software. It has been established that during the formation of resistance to disinfectants, the totality of general cellular proteins changes sufficiently to influence the results of automatic identification by mass spectrometry. All the original strains were successfully identified to the species level, and among the strains with resistance, only 40% were able to successfully identify the species. The principal possibility of using the MALDI-TOF mass spectrometry method for the identification of strains of pathogens of glanders and melioidosis with different sensitivity to disinfectants is shown.

Keywords:microbiology, resistance, mass spectrometric analysis, intracellular proteins.
 

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Citation link:
Sharov T. N., Luchinin D. N., Molchanova E. V. MASS SPECTROMETRIC ANALYSIS OF STRAINS OF GLANDERS AND MELIOIDOSIS AGENT RESISTANT TO VARIOUS DISINFECTANTS // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2024. -№11/2. -С. 61-66 DOI 10.37882/2223-2966.2024.11-2.37
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